Publikation Nr. 718 - Details
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Inter-and intramolecular fluorescence quenching of organic dyes by tryptophan
Marmé, N., Knemeyer, J.-P., Sauer, M. & Wolfrum, J. (2003). Inter-and intramolecular fluorescence quenching of organic dyes by tryptophan. Bioconjugate chemistry, 2003(14), 1133-1139.
Abstract
Steady-state and time-resolved fluorescence measurements were performed to elucidate the fluorescence quenching of oxazine, rhodamine, carbocyanine, and bora-diaza-indacene dyes by amino acids. Among the natural amino acids, tryptophan exhibits the most pronounced quenching efficiency. Especially, the red-absorbing dyes ATTO 655, ATTO 680, and the oxazine derivative MR 121 are strongly quenched almost exclusively by tryptophan due to the formation of weak or nonfluorescent ground-state complexes
