Publikation Nr. 720 - Details
SonstigesOpen AccessPrintEnglish
Specific detection of proteolytic enzymes
Marmé, N., Knemeyer, J.-P., Sauer, M. & Wolfrum, J. (2002). Specific detection of proteolytic enzymes. Patent: US 7329505 B2.
Abstract
Specific detection of proteolytic enzymes is achieved by extinguishing dye fluorescence by the amino acid tryptophan. Ttyptophan is disposed on one side of the cutting site of a proteolytic enzyme while an amino acid marked with a dye is arranged on the other side. Extinction of fluorescence occurs prior to enzyme cutting. Spatial separation of the tryptophan and the dye takes place after cutting, whereby fluorescence extinction does not occur. The dye can then fluoresce and a signal increase oc
