Publikation Nr. 725 - Details
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Novel fluorescently labeled enzyme substrates for the sensitive detection of HIV-protease
Staudt, T., Knemeyer, L., Kräusslich, H.-G., Knemeyer, J.-P. & Marmé, N. (2005). Novel fluorescently labeled enzyme substrates for the sensitive detection of HIV-protease. Proceedings of SPIE - The International Society for Optical Engineering, 2005(5704), 112-119.
Abstract
In this paper we applied the efficient fluorescence quenching of the red-absorbing oxazine derivative MR121 by the amino acid tryptophan to develop a new fluorescence based enzyme assay that can be used for detection of exopeptidases and endopeptidases. Therefore, we developed peptide substrates labeled with only one chromophore, which is quenched by a neighbored tryptophan residue via photoinduced electron transfer. The specific cleavage site for the target enzyme is located between the chromop
