Publikation Nr. 731 - Details
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Single nucleotide polymorphism analysis using different colored dye dimer probes
Marmé, N., Friedrich, A., Denapaite, D., Hakenbeck, R. & Knemeyer, J.-P. (2006). Single nucleotide polymorphism analysis using different colored dye dimer probes. Chemical Physics Letters, 2006(428), 440-445.
Abstract
Fluorescence quenching by dye dimer formation has been utilized to develop hairpin-structured DNA probes for the detection of a single nucleotide polymorphism (SNP) in the penicillin target gene pbp2x, which is implicated in the penicillin resistance of Streptococcus pneumoniae. We designed two specific DNA probes for the identification of the pbp2x genes from a penicillin susceptible strain R6 and a resistant strain Streptococcus mitis 661 using green-fluorescent tetramethylrhodamine (TMR) and
