Publikation Nr. 733 - Details
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Spectrally resolved fluorescence lifetime imaging microscopy (SFLIM) - An appropriate method for imaging single molecules in living cells
Knemeyer, J.-P., Marmé, N. & Hoheisel, J. D. (2007). Spectrally resolved fluorescence lifetime imaging microscopy (SFLIM) - An appropriate method for imaging single molecules in living cells. Analytical and Bioanalytical Chemistry, 2007(387), 37-40.
Abstract
Spectrally Resolved Fluorescence Lifetime Imaging Microscopy (SFLIM), that uses a standard confocal microscope for imaging single molecules in living cells was presented. The fluorescence lifetime images were generated by recording the signals from two avalanche photodiodes by time-correlated single-photon counting interface card. The image signal data to determine monoexponential fluorescence lifetimes from a low photon statistics, was analyzed by applying a maximum likelihood estimator (MLE) a
