Publikation Nr. 769 - Details
ArtikelOpen AccessPeer ReviewPrintEnglish
Single-molecule detection on a protein-array assay platform for detection of a tuberculosis antigen 2011, 10(3), 1316-1322
Schmidt, R., Jacac, J., Schierwitz, C., Stadler, V., Michel, G., Marmé, N., Schütz, G., Hoheisel, J. D. & Knemeyer, J.-P. (2011). Single-molecule detection on a protein-array assay platform for detection of a tuberculosis antigen 2011, 10(3), 1316-1322. J. Proteome Res., 2011(10), 1316-1322.
Abstract
Based on a single-molecule sensitive fluorescence-linked immunosorbent assay, an analytical platform for the detection of lipoarabinomannan (LAM), a lipopolysaccharide marker of tuberculosis, was established that is about 3 orders of magnitude more sensitive than comparable current ELISA assays. No amplification step was required. Also, no particular sample preparation had to be done. Since individual binding events are detected, true quantification was possible simply by counting individual sig
