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Dr. Knemeyer, Jens-Peter

Postdoc; Fak. 3 - Physik

Icon Mail knemeyer[at]ph-heidelberg.de
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Projekte

Fak. 3 • Mathematik
Projekt-ID:865
Entwicklungsvorhaben
abgeschlossen

Akronym: KI-Lehramt Entwicklung und Evaluation eines KI-Camus-Kurses (online) zur Implementierung des Themas „Ku?nstliche Intelligenz“ in die Lehramtsausbildung der MINT-Fächer
Acronym: AI teacher training Development and evaluation of a KI-Camus course (online) for the implementation of the topic “Artificial Intelligence” in teacher training for the MINT subjects
Projekt-ID:864
Transferprojekt
abgeschlossen

,Girls' Digital Camps" Transfer in den Wirtschaftsregionen Rhein-Neckar, Heilbronn­Franken und fünf weiteren Wirtschaftsregionen
,Girls' Digital Camps" Transfer
Projekt-ID:866
Forschungsprojekt
abgeschlossen

Fit for Future - Zukunftslabor MINT
Fit for Future - Lab
Projekt-ID:850
Forschungsprojekt
abgeschlossen

„Entwicklung und Evaluation eines KI-Campus-Kurses (online) zur Implementierung des Themas „Künstliche Intelligenz“ in die Lehramtsausbildung der MINT-Fächer“
„Entwicklung und Evaluation eines KI-Campus-Kurses (online) zur Implementierung des Themas „Künstliche Intelligenz“ in die Lehramtsausbildung der MINT-Fächer“
Projekt-ID:867
Entwicklungsvorhaben
abgeschlossen

IT-Berufsorientierungs-Akademien für Schülerinnen
IT career orientation academies for school girls
Projekt-ID:634
Entwicklungsvorhaben
abgeschlossen

Girls´ Digital Camps
Girls´ Digital Camps
Projekt-ID:635
Forschungsprojekt
abgeschlossen

Foresight4Youth! Arbeitswelten von morgen aus Sicht der Jugend von heute.
Foresight4Youth! Working worlds of tomorrow from the perspective of today's youth.
Projekt-ID:633
Entwicklungsvorhaben
abgeschlossen

helpING – mit MINT-Kompetenz und sozialem Engagement gesellschaftlichen Herausforderungen von morgen begegnen
Fak. 3 • Physik
Projekt-ID:865
Entwicklungsvorhaben
abgeschlossen

Akronym: KI-Lehramt Entwicklung und Evaluation eines KI-Camus-Kurses (online) zur Implementierung des Themas „Ku?nstliche Intelligenz“ in die Lehramtsausbildung der MINT-Fächer
Acronym: AI teacher training Development and evaluation of a KI-Camus course (online) for the implementation of the topic “Artificial Intelligence” in teacher training for the MINT subjects
Projekt-ID:864
Transferprojekt
abgeschlossen

,Girls' Digital Camps" Transfer in den Wirtschaftsregionen Rhein-Neckar, Heilbronn­Franken und fünf weiteren Wirtschaftsregionen
,Girls' Digital Camps" Transfer
Projekt-ID:866
Forschungsprojekt
abgeschlossen

Fit for Future - Zukunftslabor MINT
Fit for Future - Lab
Projekt-ID:850
Forschungsprojekt
abgeschlossen

„Entwicklung und Evaluation eines KI-Campus-Kurses (online) zur Implementierung des Themas „Künstliche Intelligenz“ in die Lehramtsausbildung der MINT-Fächer“
„Entwicklung und Evaluation eines KI-Campus-Kurses (online) zur Implementierung des Themas „Künstliche Intelligenz“ in die Lehramtsausbildung der MINT-Fächer“
Projekt-ID:867
Entwicklungsvorhaben
abgeschlossen

IT-Berufsorientierungs-Akademien für Schülerinnen
IT career orientation academies for school girls
Projekt-ID:634
Entwicklungsvorhaben
abgeschlossen

Girls´ Digital Camps
Girls´ Digital Camps
Projekt-ID:635
Forschungsprojekt
abgeschlossen

Foresight4Youth! Arbeitswelten von morgen aus Sicht der Jugend von heute.
Foresight4Youth! Working worlds of tomorrow from the perspective of today's youth.
Projekt-ID:633
Entwicklungsvorhaben
abgeschlossen

helpING – mit MINT-Kompetenz und sozialem Engagement gesellschaftlichen Herausforderungen von morgen begegnen

Publikationen

Alle2019201820172016201520142011201020082007200620052004200320022000
ArtikelOnlinePeer ReviewPrintEnglish

A fluorescence-based assay for exopeptidases using self-quenching peptide probes with single-molecule sensitivity

Marmé, N., Weston, K. D., Staudt, T., Spatz, J. & Knemeyer, J.-P. (2005). A fluorescence-based assay for exopeptidases using self-quenching peptide probes with single-molecule sensitivity. International Journal of Environmental Analytical Chemistry, 2005(85), 741-751.

  • Linkresearchgate.net/publication/...
  • StichworteIn recent years, the interest in assaying exopeptidases has become increasingly important because they are significantly involved in many diseases like cancer. To date, no generally applicable fluorescence-based detection method has been developed because
Noch kein Titelbild vorhanden
ArtikelOnlinePeer ReviewPrintEnglish

A new assay for single nucleotide polymorphism analysis based on displacement reactions in PNA–DNA double helices

Habl, G., Böhm, H., Marmé, N. & Knemeyer, J.-P. (2005). A new assay for single nucleotide polymorphism analysis based on displacement reactions in PNA–DNA double helices. International Journal of Environmental Analytical Chemistry, 2005(85), 613-623.

Abstract
In this article, we describe a new method for single nucleotide polymorphism analysis using the displacement reaction of the DNA in a PNA–DNA double helix by the target DNA. Thereby, the probe consists of a TMR-labeled PNA and a Cy5-labeled DNA forming a FRET system. Due to the displacement of the labeled DNA strand by the target DNA, the FRET is invalidated and the fluorescence of the donor dye (TMR) increases. Investigations of the exchange reaction show that increasing salt concentratio
Noch kein Titelbild vorhanden
ArtikelOnlinePeer ReviewPrintEnglish

Aggregation behavior of the red-absorbing oxazine derivative MR 121: A new method for determination of pure dimer spectra

Marmé, N., Habl, G. & Knemeyer, J.-P. (2005). Aggregation behavior of the red-absorbing oxazine derivative MR 121: A new method for determination of pure dimer spectra. Chemical Physics Letters, 2005(408), 221-225.

Abstract
In this Letter, we investigated the dimerization behavior of the red-absorbing oxazine derivative MR121. Thereby a new method to determine absorption spectra of pure non-fluorescent H-type dye dimers is presented. Therefore, the amino acid lysine is doubly labeled with the fluorescent dye in order to obtain an absorption spectrum mainly caused by dimers. By subtracting the fraction arising from monomeric dye, the dimer spectrum is generated. The extinction coefficient at absorption maximum (608n
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ArtikelOnlineohne Peer ReviewPrintEnglish

Novel fluorescently labeled enzyme substrates for the sensitive detection of HIV-protease

Staudt, T., Knemeyer, L., Kräusslich, H.-G., Knemeyer, J.-P. & Marmé, N. (2005). Novel fluorescently labeled enzyme substrates for the sensitive detection of HIV-protease. Proceedings of SPIE - The International Society for Optical Engineering, 2005(5704), 112-119.

Abstract
In this paper we applied the efficient fluorescence quenching of the red-absorbing oxazine derivative MR121 by the amino acid tryptophan to develop a new fluorescence based enzyme assay that can be used for detection of exopeptidases and endopeptidases. Therefore, we developed peptide substrates labeled with only one chromophore, which is quenched by a neighbored tryptophan residue via photoinduced electron transfer. The specific cleavage site for the target enzyme is located between the chromop
Noch kein Titelbild vorhanden
ArtikelOnlineohne Peer ReviewPrintEnglish

Self-quenching DNA probes based on aggregation of fluorescent dyes

Schäfer, G., Müller, M., Häfner, B., Habl, G., Nolte, O., Marmé, N. & Knemeyer, J.-P. (2005). Self-quenching DNA probes based on aggregation of fluorescent dyes. Proceedings of SPIE - The International Society for Optical Engineering, 2005(5704), 30-39.

Abstract
Here we present a novel class of self-quenching, double-labeled DNA probes based on the formation of non fluorescent H-type dye dimers. We therefore investigated the aggregation behavior of the red-absorbing oxazine derivative MR121 and found a dimerization constant of about 3000 M-1. This dye was successfully used to develop hairpin-structured as well as linear self-quenching DNA probes that report the presence of the target DNA by an increase of the fluorescence intensity by a factor of 3 to 1
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ArtikelOnlinePeer ReviewPrintEnglish

Self-quenching DNA probes based on dye dimerization for identification of mycobacteria

Knemeyer, J.-P., Marmé, N., Häfner, B., Habl, G., Schäfer, G., Müller, M., Nolte, O., Sauer, M. & Wolfrum, J. (2005). Self-quenching DNA probes based on dye dimerization for identification of mycobacteria. International Journal of Environmental Analytical Chemistry, 2005(85), 625-637.

Abstract
This paper presents new self-quenching DNA probes that exploit the efficient fluorescence quenching by the formation of dye dimers. The probes consist of a hairpin-structured oligonucleotide that is labeled with two identical fluorescence dyes that are able to form non-fluorescent H-type dimers while the hairpin is closed. We used the oxazine derivative MR 121 that has a sufficient dimerization tendency and can be excited by a pulsed diode laser emitting at 635 nm. Upon hybridization to t
Noch kein Titelbild vorhanden